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rat anti ne  (R&D Systems)


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    Structured Review

    R&D Systems rat anti ne
    Rat Anti Ne, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+mouse+ne+ela2+antibody/Mouse+Neutrophil+Elastase%2FELA2+Antibody/pmc13038209-176-63-65
    Average 94 stars, based on 20 article reviews
    rat anti ne - by Bioz Stars, 2026-09
    94/100 stars

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    Article Title: A TLR–CXCL1 pathway in DRG neurons induces neutrophil accumulation in the DRG and mechanical allodynia in EAE mice
    Article Snippet: After transfer, the membranes were blocked with TBS-T (0.2% Tween-20 in TBS) containing 5% Blocking One (Nakarai Tesque, Kyoto, Japan) for 1 h at room temperature, and then incubated with the primary antibodies diluted in TBS-T containing 5% Blocking One for overnight at 4 °C. .. The following primary antibodies were used: anti-myeloperoxidase antibody (1:2,000; cat. no. AF3667, R&D Systems), anti-mouse NE/ELA2 antibody (a marker for NE, 1:2,000; AF4517, R&D Systems), anti-CXCL1 antibody (1:2,000; cat. no. AF453, R&D Systems), anti-TLR4 antibody (1:2,000; cat. no. 19811-1-AP, Proteintech), and anti-β-actin antibody (1:10,000; cat. no. ab8226, Abcam). .. After being washed with TBS-T, the membranes were incubated with horseradish peroxidase-conjugated secondary antibody (1:1,000; GE Healthcare) for 2 h at room temperature.



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